Faiez Alani
McMaster University, Canada
Title: Optimization of lipase production by solid state bioprocess using packed bed bioreactor
Biography
Biography: Faiez Alani
Abstract
Lipase catalyzes hydrolysis of lipids to diglycerides and monoglycerides, fatty acids and glycerol. Demand on lipase application in food, detergent, pharmaceutical and biodiesel industries was increased in last decade. We are reporting novel process for lipase production. Solid state bioprocess was used as production technique in packed bed bioreactor. Distiller’s dried grains with solubles (DDGS); a co-product from ethanol industry was used as a substrate for lipase production in this process. Penicillium restrictium produced more lipase than Y. lipolytica or R. miehei. It was found that optimum conditions for lipase production were 70% moisture content, pH 4.5 and 8 days incubation time. Scale-up of lipase production by using packed bed bioreactor increased significantly lipase production.