Omirbekova N Zh
Al-Farabi Kazakh National University, Kazakhstan
Title: Development of approach to obtain Brachypodium distachyon L. regenerative plants with morphogenetic stability
Biography
Biography: Omirbekova N Zh
Abstract
The aim of the research is development of eff ective methodological approaches of in vitro cultivation, object 21 line (BD21) B.
distachyon. In order to develop cultivation methods, ability for callus formation, regeneration of generative and vegetative organs
of VD21 was studied. To cultivate, Linsmayer-Skoog and Murashige-Skoog medium, additional introduction of phytohormones
was used. Aseptic culture conditions for callusogenesis cultivation: under dark conditions at a temperature of 24°C, for t shoots
regeneration: 16/8 hour photoperiod and lighting of 3000 lux. Infl orescence and immature embryos isolated from green spikes of
vegetating plants and isolated embryos from mature seeds were used as primary explants to induce callus formation in vitro. During
immature embryo cultivation, callus formation takes place near the corimbe for 20-25 days. During the cultivation of whole caryopsis
with mature embryos, the sprouts grew aft er a week of cultivation on MS medium without hormones. Th e level of maturity of isolated
caryopsis has a signifi cant infl uence on the callus formation and the type of callus tissue. Th e mature caryopsis formed callus on
the 10th day of cultivation with a frequency of 75%. Th e cultivation of the overgrown caryopsis in the dark on medium MS 1 with
2 mg/L 2.4 DPA, led to the formation of a primary shoot in 60% of explants; the formation of callus in the area of the scute, but for
30-35 days. Passage of the callus on the same medium and on the hormone-free medium led to the appearance of greenish pointwise
impregnation of 30% of the calluses. For microclonal propagation, nodal segments of young shoots of plants were introduced into the
culture. To culture introduction, side shoots 5 cm long with 3-4 interstitial sites were cut, the microcrops were planted in inducing
media. Th e shoot-forming capacity of primary explants was about 59%; the multiplication factor for two passages was 5.7.