24th Global Congress on Biotechnology
University of São Paulo, Brazil
Title: Inoculation methods for introducing the endophytic fungi Mucor nidicola in tomato plants, and its effect on promoting resistance to cadmium
Biography: Helena Gutierrez Oliveira
Soil pollution caused by heavy metals is a global concern that has been increasing due to industrial development and mining residues. Bioremediation is the use of living organisms to remove or neutralize pollutants from a contaminated site. Among the possible strategies, phytoremediation is the direct use of green plants and their associated micro-organisms to stabilize or reduce contamination in soils. This study analyzed the inoculation efficiency of fungus Mucornidicola in tomato Solanumlycopersicum and its role in plant resistance to heavy metals. The fungus tested was isolated from the roots of plants from a site contaminated with heavy metals and it showed good resistance to cadmiun when tested in vitro. First, we tested nine methodologies for the fungus inoculation on tomato plants. Then, the plant-fungus interaction was studied in the presence of cadmium to analyze the fungus as a plant resistance promoter. The inoculation techniques that applied short-time chemical scarification were not efficient and neither were the methodologies that placed a disk of the mycelium with the seed during the germination process. Spraying the plants with mycelium solution was also inefficient. The long-term chemical scarification and direct contact between seeds and sporum resulted in fungal inoculation, even though it occurred at a low frequency. The plant-fungus interaction test in presence of CdCl2 confirmed the negative effect of this compound on plant development, but there was no significant effect of fungus inoculation in terms of improving plant performance in such conditions. Further research on the improvement of the inoculation technique with M. nidicola on tomato or other species is of great importance to enable the development of new viable phytoremediation techniques.