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Magaji Sanusi

Magaji Sanusi

Department of Science Laboratory, Nigeria

Title: Problems associated with small scale plasmid DNA isolation, restriction digestion and agarose gel electrophoresis

Biography

Biography: Magaji Sanusi

Abstract

Two experiments on alkaline lysis method of plasmid DNA isolation was carried out utilizing strains of E.coli JM109, a non- pathogenic bacteria that has been deliberately disabled. Restriction digests and gel electrophoresis carried out. The result of the first gel electrophoresis reveals only traces of RNA, hence no DNA fragments on the samples loaded labeled A to D this could be attributed to possible contamination from the procedures. For the second gel electrophoresis samples, A is plasmid free, B and C contains small fragments approximately 2.3 to 5.6 and D contains the fertility plasmids.