A Vikulina has completed her PhD in the field of Biological Science in Lomonosov Moscow State University, Russia. Currently, she is Marie-Curie Fellow in Fraunhofer Institute for Cell Therapy and Immunology, Potsdam, Germany. Her research is focused on the development of drug delivery carriers for controlled drug delivery and testing as well as for deciphering the pathways of biological action and transport of drugs. She has been awarded by prestigeous Alexander Von Humboldt and Marie-Curie Fellowships, served as a member of organizing committees at international conferences and scientific olympiads. She is also a Guest Editor in Micromachines Journal.
Biopolymer based multilayer capsules are novel vectors for advanced drug delivery. Capsules assembled using decomposable and mesoporous CaCO3 vaterite crystals can host enormous amounts of biomolecules (such as proteins and peptides, small drugs, nucleic acids, etc1) and release them in a controlled manner. Protection and controlled release of biomolecules are the main advantages of the capsules; this can be achieved by adjusting the capsule structure by varying the number of layers, polymer nature and distribution into capsules. Loading of biomolecules into capsules at physiologically relevant and mild conditions is indispensable for bio-applications. This work aims to fabricate capsules from a variety of biopolymers and assess their stability and the encapsulation performance. The following biopolymers have been tested for polyanions (chondroitin sulfate, hyaluronic acid, dextran sulfate, and heparin) and polycations (poly-L-lysine, dextran amine, collagen, and protamine). The most attractive pairs of biopolymers, in-terms of capsule integrity are identified and retention of biomolecules within the capsules is considered. Shrinkage of capsules at room temperature during CaCO3 removal is used for capture of biomolecules into capsules and is discussed by taking into account the charge compensation in cooperative interpolymer complexation. Interestingly, occupation of the vaterite crystal pores with polymer during capsule fabrication is also responsible for the observed capsule shrinkage and fusion phenomena
Martin Schomber has completed his Master studies in pharmaceutical biotechnolgy at the age of 25 years from Technische Hochschule Mittelhessen. In his studies, he has been a part of different research work groups in molecular, enzymantical and industrial parts of biotechnology. During a research stay at the University of Auckland (New Zealand), he was involved in a methodical studie to identify beta-1,3 glucan in wood for specific identification of different kinds of wood for industrial application.
Bioethanol is produced in general by starch fermentation from corn or sugar beets. An alternative is the use of lignocellulose by digesting cellulose to glucose. This digestion is an enzymatic process in which endocellulases, cellobiohydrolses and β-glucosidase, named as cellulase complex, are interacting together. The most common used fungal strain for cellulase production, Trichoderma reseei, has been studied intensively and optimized in recent years. The metabolism of cellulase production has been described in different literatures for fungis but is not exactly understood so far. The problem of cellulase production by Trichoderma reseei is based on a not well balanced enzyme complex. There is a low production rate of beta-glucosidase in this fungi that leads to the addition of the minor enzyme in industrial scale fermentation. Our studies focuses on a cellulase production with Penicillium verruculosum mutants that has a more balanced cellulase complex. Beech wood is used as lignocellulose substrate in this study which is pretreated by organosolv process technology for separation of hemicellulose, lignin and cellulose. This poster will present first fermentation results by using different substrates and varying fermentation methods to optimize the enzyme production in both, lab and pilot scale.