Day 2 :
Rice University, USA
Oara Neumann is the Peter M and Ruth L Nicholas Research Scientist at Rice University (a fully funded, endowed research scientist position at the university). She has completed her PhD and Postdoctoral study in Applied Physics at Rice University, MSc in Chemical Physics at Weizmann Institute of Science, Israel and another MSc in Analytical Chemistry from Bucharest University, Romania. She is the Pioneer of nanoparticle-based solar thermal applications. She holds several patents and has published more than 25 refereed articles and has an h-index of 16.
Engineering a compact, near-infrared plasmonic nanostructure with integrated image enhancing agents for combined imaging and therapy is an important nano-medical challenge. To overcome this challenge we designed a nanostructure with NIR plasmonic signatures composed of a 50 nm Au core surrounded a SiO2 inner-shell doped with contrast agents and an outer Au shell. The plasmon resonance of this nanostructure known as a nano-Matryoshka (NM), can be tuned to the desired wavelength by varying the thickness of the layers. The encapsulated contrast agents used in this study are Fe(III)-DOTA, Gd(III)-DOTA and fluorescent dyes. The Fe(III)-NM based contrast agents are found to have relaxivities two times greater than the widely used Gd(III)-DOTA, providing a practical alternative for T1 MRI contrast agent that eliminates Gd(III) patient exposure entirely. Additionally, the internalization of fluorescent dyes and MRI contrast imaging agents within the NM substantially reduces the toxicity while maintaining a free nanoparticles surface for further bio-functionalization.
AmpTec GmbH, Germany
Keynote: Synthetic mRNAs in clinical trials: Manufacturing of high quality GMP-grade synthetic mRNAs
Time : 10:30-11:00
Guido Krupp is a CEO and President of AmpTec GmbH. He received his PhD Degree from Würzburg University and Max-Planck-Institute Martinsried in 1981. He was Postdoc at Yale University from 1983 to 1987. He was a Research Group Leader at Kiel University from 1987 to 2002 and Founder of artus GmbH, 1998 and AmpTec GmbH, 2005 and KSK Diagnostics GmbH, 2015. His research interests include nucleic acid technology with focus on RNA, plant pathogens (viroids), ribozymes and telomerase. He has more than 60 publications, Editor of “Ribozyme Biochemistry and Biotechnology” and Editorial Board of “Biotechnology Annual Review”.
Synthetic mRNAs from AmpTec have achieved a world-wide acceptance. AmpTec’s mRNAs are available as GMP grade products and have been introduced in several clinical trials in Europe, Australia and USA. Availability of high quality synthetic mRNAs is crucial in enabling significant progress in this field. Worldwide, a very limited number of active manufacturers of high quality mRNAs, AmpTec continues to realize its obligation to support the entry of new players by providing customized, mRNA products at small and large scales, from mg to grams. Applications of synthetic mRNA include reprogramming of human cells; antigen expression for vaccination projects in oncogenesis, infectious disease and allergy prevention; protein-replacement therapies. In a recent overview, applications and corresponding synthetic mRNA quality requirements were presented by Quabius & Krupp in New Biotechnology 2015. Syn-mRNAs can be generated by in vitro transcription (IVT) from defined templates containing the synthetic gene of interest. Optimal mRNA activity depends on a long, unmasked poly(A) tail, but long hompolymeric sequence are not reliably propagated in E.coli. Our alternative procedure uses PCR products as IVT-templates resulting in very well defined and easily modified poly(A) tails. Possible problems: Challenging sequences can lead to poor results in generation of the PCR template (AmpTec workflow) or (ii) during in vitro transcription reactions (workflow of all current mRNA manufacturers). For both steps (i) and (ii), results and trouble shooting are presented. Quality requirements and QC methods for GMP-grade synthetic mRNAs in therapeutic applications are presented.
Fraunhofer Institute for Cell Therapy and Immunology, Germany
Anna S Vikulina has completed her PhD in the field of Biological Science in Lomonosov Moscow State University, Russia. Currently, she is Marie-Curie Fellow in Fraunhofer Institute for Cell Therapy and Immunology, Potsdam, Germany. Her research is focused on the development of drug delivery carriers for controlled drug delivery and testing as well as for deciphering the pathways of biological action and transport of drugs. She has been awarded by prestigeous Alexander Von Humboldt and Marie-Curie Fellowships, served as a member of Organizing Committees at international conferences and scientific olympiads. She is also a guest editor in Micromachines Journal.
Now-a-days sequential deposition of naturally derived and oppositely charged biopolymers known as the layer-by-layer (LBL) technology became one of the key modern strategies for generating functional biomaterial coatings for diverse applications such as tissue engeenering, implant coatings and drug delivery. This was largely driven by the power of the LBL approach for biomimetics of extracellular matrix with a high precision at the nanoscale. The LBL technique has been also combined with a variety of soft and hard species including nanoparticles, carbon nanotubes, lipid bilayers in order to endow these hybrid materials with unique properties. More recently the LBL technology has been developed towards the coating of peculiar templates ranging from soft biomaterials (emulsions, liposomes and biological cells) to hard cores of sofisticated geometris (graphene, nanoparticles, inorganic crystals and their assemblies). This talk will focus on the design and applications of hybrid biomaterials made up taking advantage of the LBL approach. Among the variety of unconventional assemblies and architectures, coupling of the LBL coating with lipid and polymeric structures (soft), gold nanoparticles (hard-on-soft) and vaterite calcium carbonate crystals (hard) will be considered. Passive and active (temperature triggered) molecular transport within the LBL assembled structures will be addressed. Perspectives of the use of these hybrid assemblies will be highlighted.