24^th Global Congress on Biotechnology June 15-16, 2020 London, UK

Biography:

In 2004, Marina Sidorenko has completed his PhD in two specialties: soil science and microbiology. She is a leading researcher at the FSCEATB FEB RAS. She has published over 35 articles in well-known journals and 4 patents of the Russian Federation, is a member of the editorial board and reviewer of several journals. Her areas of interest are applied microbiology, soil microbiome, sanitarymicrobiology.Vladislav Sidorenko is a student of PRMU. He has a 3 articles in scientific journal. Hers scientific interests: medical microbiology, epidemiology, infectious diseases.

Abstract:

Listeria monocytogenes is the causative agent of human and animal listeriosis. It is known that the classical bacterial forms of Listeria, being saprophytes, have high stability in the external environment, they are able to reproduce in various environmental objects, including in soils and plants. It is known that plants can be a natural reservoir of pathogenic Listeria and a source of human infection. Volatile organic emissions of germinating seeds, due to the high penetrating ability in the soil, availability for assimilation, can be a source of carbon and energy for soil microorganisms. It is known that not all bacteria are capable of assimilating the volatile metabolites of germinating seeds, and the specificity of the action of volatile organic compounds depends on both the type of bacteria and the type of germinated seeds. Therefore, the volatile metabolites of germinating seeds of cultivated plants were studied, which are factors of transmission of L. monocytogenes - lettuce (Zactuca sativa), corn (Zea mays L.). As a result, it is proved that the volatile metabolites of germinating plant seeds stimulate the growth and reproduction of pathogenic listeria in soils. The specificity of the action of volatile organic compounds of plants on the reproduction of the studied bacteria is noted. The main volatile fraction that affects the growth and reproduction of L. monocytogenes is methanol, which bacteria use as their sole source of carbon and energy.

Biography:

DeepikaRamachandra graduated from medical school, Bangalore Medical College. She has been extensively involved in the field of research, has presented in numerous international conferences, awarded Best Paper for her previous presentations. She has received prestigious International Award for Young People by HRH Duke of Edinburgh. She is also a national level athlete with numerous records held in her name. She is also member of various NGO’s and involved in a lot of philanthropy both in India and US.

Abstract:

Plastic is convenient, lightweight, unbreakable and relatively inexpensive. However, there are both environmental and health risk from the widespread use of plastics. BisphenolA (BPA), a chemical that mimics the action of the human hormone estrogen, can leach from polycarbonate plastic. Bisphenol A has been found to stimulate prostate cancer cells7 and causes breast tissue changes in mice that resemble early stages of breast cancer in both mice and humans. Reusable bags for transport of groceries from the store to the consumer’s home have become popular in recent years. Since these bags are often reused, and used potentially for multiple purposes, the possibility for contamination of food products as well as the consumer’s hands. Most food borne illnesses are believed to originate in the home. Reuse of bags creates an opportunity for cross contamination of foods. Study was to assess the potential for cross contamination of food products from reusable bags used to carry groceries. It is recommended that the public needs to be educated about the proper care of educated about the proper care of reusable bags by printed instructions on the bags or through public service announcements improper cooking or handling of foods. Reusable bags if not properly washed between uses, create the potential for cross contamination of foods. The common use of bags for other purposes than carrying groceries is also a potential concern. Thus, a sudden or significant increase in use of reusable bags without a major public education campaign on how to reduce the risk of cross contamination would create the risk of significant adverse public health impacts. Shoppers who do not regularly wash their reusable grocery bags may be placing themselves and their families at heightened risk of food borne illness. Reusable bags, if not properly washed between uses, create the potential for cross-contamination of food. This risk can be increased by the growth of bacteria in the bags. Fortunately, washing the bags by machine or hand reduced bacterial presence to nearly zero. Reusable grocery bags can be a breeding ground for dangerous food-borne bacteria and pose a serious risk to public health. Bacteria levels found in reusable bags were significant enough to cause a wide range of serious health problems and even death. They are a particular danger for young children, who are especially vulnerable to food-borne illnesses. Antibiotic sensitivity test was performed on all bacterial isolates against 15 antibiotics (representing different class of antibiotics) by Kirby Bauer’s disc diffusion method on Mueller Hilton agar by using CLSI guidelines. Zones of incubations at 37 C to nearest millimeter with a slide gauge.

Biography:

K. M. Yacobis a practicing physician in the field of healthcare in the state of Kerala in India for the last 30 years and very much interested in basic research. His interest is spread across the fever, inflammation and back pain. He is a writer. He has printed and published nine books on these subjects. He wrote hundreds of articles in various magazines.After scientific studies, He have developed 8000 affirmative cross checking questions. It can explain all queries related to fever

Abstract:

We have been hearing for centuries that ‘fever is not a disease but a symptom’. Physicians say that fever is a symptom of diseases like flu to cancer.The conservative fever definition, diagnosis, and treatments are based on fever as a symptom.All the studies related to fever as a symptom of a disease have been done without knowing the Purpose of the temperature of fever is. Without knowing the Purpose of the temperature of fever, how can fever included in the symptom definition? Temperature between 38o to 41o centigrade can be symptom of a disease? Most of the diseases may not have a fever. Sometimes it disappears. Then, is fever a symptom of which disease? Symptom Definition is the only parameter necessary for a Symptom. As with any or all other definitions, symptom definition should describe the symptom scientifically. If it cannot describe clearly, there is no use of a symptom definition. A symptom is a departure from normal function or feeling which is noticed only by a patient, indicating the presence of disease or abnormality.

In fever, both symptoms of disease and symptoms of Fever are included. Deduct symptom of disease from total symptoms, we will get symptoms of fever. In fever does not show any actions of temperature rise. How can we prove the fever is not a symptom? The fever is not symptom when examined in various directions.   In fever, both symptoms of disease and symptoms of fever are included. Deduct symptom of disease from total symptoms, we will get symptoms of fever. We can separate signs, signals, and actions of both fever and disease and rising temperature. Temperature between 38 degrees and 41 degrees cannot be a symptom of any of the diseases.  A different cause of diseases like virus, bacteria, fungi, venom, horror scene, and horror dream never shows the same symptoms.

Fever has never been scientifically proved as a symptom of a disease. Fever has the properties of adaptation. If we ask any type of question-related to fever by assuming that the fever is not a symptom we will get a clear answer. If we avoid or evade from this we will never get a proper answer to even a single question.

Biography:

Hamza A. Aboeleninis an Experienced Quality control Microbiologist and researcher with a demonstrated history of working in the pharmaceuticals industry Strong research professional with a Bachelor of Science (BS) focused in Environmental Science & Microbiology from Alexandria University.

Abstract:

The validation of the disinfectants in the pharmaceutical industry environment is essential process to be sure of the disinfection process of it.The Aim of the study is to determine the efficacy of Dettol disinfectant in compare to the used disinfectants (chlorine – phenol – alcohol) on different surfaces (Epoxy – stainless steel – glass) in the production environment

Four concentration (5,2.5,1.25and.625 %) of Dettol was compared to chlorine 1% and phenol 5% and alcohol 70% in time intervals 5, 10,15and 30 minutes  and approached to Bacillus subtilis ATCC 6633, Escherichia coli ATCC 8739, Staphylococcus aureus ATCC 6538, Pseudomonas aeruginosa  ATCC 9027, Aspergillusniger ATCC 16404 and Candida albicans ATCC 10231 using suspension test .Then the most significance concentration of Dettol and in the shortest contact time compared to the other disinfectants in the shortest contact time using surface test on different surfaces (Epoxy – stainless steel – glass) in the production environment. The efficacies calculated by log reductions are calculated according to the following equation: Log10 reduction (R) = log10 pre-value cfu/plate– log10 post value cfu/plate. A sample of four replicates were used per organism per concentration at different time intervals to estimate an overage change at log reduction equal 2 units with estimated standard deviation (SD)equal 0.5 for each sub group, using  α error equal 0.05 will provide a power of 20% . The results elaborated that the most significance concentration of Dettol is 2.5% in contact time 5 minutes and chlorine 1% in contact time 10 min phenol5% in contact time 10 min alcohol 70%in contact time 10 min. 

Biography:

Paula Istvan has completed his PhD from University of Brasilia, Brazil in Biotecnology and she is doing postdoctoral studies in the Ben-Gurion University of the Negev, Israel. She has experience in recombinant protein purification process development, molecular biology, enzymology and bioinformatics. A comprehensive intercultural and international experience (Romanian with and PhD in Brazil doing PD in Israel).

Abstract:

The RDX, hexahydro-1,3,5-trinitro-1,3,5-triazine, is an explosive, extensively used by the military and in the construction industry. It poses harmful and deleterious threats to the environment and causes soil, sediment, and groundwater contamination. The microorganisms involved in RDX biodegradation are limited by a small fraction of RDX isolates, because approximately 99% of microorganisms are uncultivable under laboratory conditions. Using a cultivation-independent molecular technique as sequencing is necessary to probe the insight into RDX-degrading microorganisms. Rhodococcus YH1 and Rhodococcus T7 and Gordonia YY1 stains were isolated from contaminated environments and cultivated with RDX as sole nitrogen source. Genomic DNA was extracted from a single colony and sequencing was performed using an Illumina Miseq2v-500 to generate 250x2paired-end reads. Raw sequence data were quality trimmed, and de novo  assembly was performed in the software package Spades. Prokka tool was used to annotate the genome using the UniProt database. Plasmid Spades program from Spades package were used for plasmid assembly. The draft genome of all stains is approximately 6 Mb, distributed in more the 200 Scaffolds. The average coverage was approximately 35X. The annotation identified unique genes, coding sequences, transfer-messenger RNA, transfer RNAs and Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR). The functional information was analyzed both individually and as in metabolic networks generated by KEGG Pathway Maps. This genomes analyses will improve our knowledge of RDX degradation genes and metabolic pathways and contribute to the development of more efficient bioremediation of polluted environments.

Biography:

Sanusi Magaji is a carrier academic with special interest in biological sciences specifically molecular biology, health sciences and bioinformatics. Born 1979 in Bauchi city of Nigeria, Sanusi holds a bacheclor of technology from ATB University Bauchi, PGD public health ATB Bauchi, Msc Molecular Biology with Bioinformatics University of Wolverhampton. Sanusi is a lecturer at the department of science lab. Tech. ATAPOLY Bauchi Nigeria. Sanusi has a special interest on bioinformatics analysis of CCR5 in HIV therapeutics.

Abstract:

Two experiments on alkaline lysis method of plasmid DNA isolation was carried out utilizing strains of E.coli JM109, a non- pathogenic bacteria that has been deliberately disabled. Restriction digests and gel electrophoresis carried out. The result of the first gel electrophoresis reveals only traces of RNA, hence no DNA fragments on the samples loaded labeled A to D this could be attributed to possible contamination from the procedures. For the second gel electrophoresis samples, A is plasmid free, B and C contains small fragments approximately 2.3 to 5.6 and D contains the fertility plasmids.

Biography:

Helena Gutierrez Olivera is a PhD candidate at Centro de EstudosNuclearesnaAgricultura,  Universidade de São Paulo, Brazil. She completed her masters at the age of 24, on agricultural enthomology at UniversidadeEstadualPaulista, Brazil. Currently, she also works as a biology teacher at hightshool level.

Abstract:

Soil pollution caused by heavy metals is a global concern that has been increasing due to industrial development and mining residues. Bioremediation is the use of living organisms to remove or neutralize pollutants from a contaminated site.  Among the possible strategies, phytoremediation is the direct use of green plants and their associated micro-organisms to stabilize or reduce contamination in soils. This study analyzed the inoculation efficiency of fungus Mucornidicola in tomato Solanumlycopersicum and its role in plant resistance to heavy metals. The fungus tested was isolated from the roots of plants from a site contaminated with heavy metals and it showed good resistance to cadmiun when tested in vitro. First, we tested nine methodologies for the fungus inoculation on tomato plants. Then, the plant-fungus interaction was studied in the presence of cadmium to analyze the fungus as a plant resistance promoter. The inoculation techniques that applied short-time chemical scarification were not efficient and neither were the methodologies that placed a disk of the mycelium with the seed during the germination process. Spraying the plants with mycelium solution was also inefficient. The long-term chemical scarification and direct contact between seeds and sporum resulted in fungal inoculation, even though it occurred at a low frequency. The plant-fungus interaction test in presence of CdCl₂ confirmed the negative effect of this compound on plant development, but there was no significant effect of fungus inoculation in terms of improving plant performance in such conditions. Further research on the improvement of the inoculation technique with M. nidicola on tomato or other species is of great importance to enable the development of new viable phytoremediation techniques.

Biography:

I am a brand strategist and passionately believe brands that serve a meaningful purpose in our lives have the ability and power to create real systemic change in our world.
 

I am currently working on a social change platform that I am developing as a brand. My goal is to bring the science of how we experience a quantum reality through our awareness into a brand expression that can be accessed by mainstream global culture. My objective is to influence our understanding of what it means to maintain a quantum mindset that experiences possible reality out of infinite possibilities, over experiencing probability as we experience reality. Bringing this understanding to education through new pedagogy and curriculum is my ultimate goal.

I have published over 25 papers that explore how we experience a quantum Universe to consider the connection between relativity and quantum theory. My work has been read in over 90 countries.

Abstract:

My work explores this reality as a whole vibration where we only experience 5% of it as normal matter. Which means we are resisting 95% of the vibration in how we experience reality in terms of how we experience the waves of information as matter. In other words, we are not experiencing constructive interference where we are amplifying the energy within the waves, we are experiencing deconstructive interference where we are cancelling the information of the waves out as we are experience reality, which disconnects the waves. Our resistance to the whole vibration is causing probable states of reality over possible states of reality that are relative to what the whole vibration is experiencing as one organism within 100% of this reality. As “free radicals” our energy is being used to maintain the velocity of our particles experiencing the additional volume of expanded space as probable states of reality, over experiencing possible states of reality relative to what the whole vibration is simultaneously experiencing.

My work explores the idea that similar to nature, we have the ability to release our electrons to form a harmonized standing wave of vibration to the whole vibration in order to become aware of possible reality that maintains relativity within the whole vibration through connected waves of matter as information. And it is the resistance to the whole vibration by only experiencing 5% of the whole vibration that “disconnects” the waves and also causes molecular disorder in our physical bodies. My work applies to biotechnology in the sense that I believe there are ways technology can assist us to measure if and when we insulate our electrons over releasing them to maintain being a complete circuit of information within singularity as it relates to our experience of the the field as reality. I believe technology can also assist us in measuring how divided our awareness is from the whole vibration as we experience reality through the rate of flow of electromagnetic energy we allow through our daily experience of reality. Maintaining relativity is learning to be the whole organism but allowing yourself to be your own unique variable within how you experience your reality. Your energy is then being used efficiently within your own awareness as any state of matter within the whole vibration and you experience more homeostasis within the systems of your own physical body by maintaining enthalpy within the whole vibration.

Biography:

Abstract:

Inefficient use of nitrogen and phosphorus leads to anthropogenic eutrophication of rivers, lakes, and oceanic basis worldwide, causing an environmental problem that can trigger a death cycle of an entire water body. Microalga, oneof the organisms that benefit from the excessive nutrient runoff and uses the sunlight to catalyze reactions that cause eutrophication, can also be part of a solution. The study presented in this thesis represents a possible integrated solution for the nutrients accumulated, especially in dairy farms. This work shows how it is possible to treat dairy wastewater in large volumes, using plastic photobioreactors with an initial inoculum of microalgae, in this case, Chlorella sp., in a mixotrophic solution (since the dairy wastewater used in this study was not sterilized). It was also shown that in pilot-scale, ratio 1:10 (dairy wastewater in water) was capable of removing high amounts of nutrients, up to 97.55% of ammonium, 39.27% of nitrate, and 27.05% of phosphate. The 1:10 was also capable of producing competitive biomass amounts when comparing to the controls, 1.575 ± 0.599 g/L and 1.315 ± 0.240 g/L Moreover, none of the treatments (control, controlN, 1:10, 1:10N, 1:30 and 1:30N) were significantly different from each other, considering the nutrients ( ,  ,  ) removal rates and biomass, when adding or not extra CO2. Besides, a study was carried to evaluate the taste preference of calves fed Chlorella sp. produced in the previous steps. Sterilized biomass was used for feeding trials with six Holstein and crossbred dairy heifer calves. No mycotoxins were found in the biomass, and many heavy metals were tested, having the levels below the maximum content recommended for animal feeding. The microalgae biomass produced had a protein content of 49.2%, 2.32% of fat, 38.5% of carbohydrates, and around 10% of different minerals and nutrients. They were fed 0, 30, and 60 g of Chlorella sp. daily in a sequential elimination study. No difference was found for dry matter intake of calves fed 0, 30, or 60 g of Chlorella sp., indicating that microalgae may be added to the rations of calves without any adverse effects.